Whole Body Cryopreservation
Consent for Cryopreservation

• Make local arrangements with the hospital or GP. We will deal with the hospital, etc regarding jumping through the necessary hoops
• Consult with medical staff and aim to deploy 24-48 hours before death is expected
• It is important to avoid death becoming reportable to the coroner where possible. If death is expected and a patient is under continuous medical care, the coroner is not usually involved. However, deaths are reportable, for example, if they are unexpected, unattended, accidental, the cause is unknown or the patient hasn't seen their doctor/consultant within the previous 14 days. If a death is reportable, there are steps we can take to mitigate the situation.
• The fact of death needs to be confirmed by the doctor/hospital in order for them to be able to issue a cause of death certificate. There is no legal requirement for the certificate to be issued or death registered before we begin cryonics procedures. However, most hospitals require the certificate to be issued before we leave the hospital.
• Upon clinical death, maintain cardio-respiratory support and administer medications to stabilise biochemistry. Transfer the patient to a private ambulance, begin cooling in an ice bath for 2 to 3 hours after death. This reduces their core temperature to between 10 and 20 degrees centigrade. Once at this temperature, metabolic demand his substantially reduced and we are able to remove the patient from cardiac respiratory support to carry out perfusion.
• A local mortuary facility (which serves as a surgical location for cryoprotective perfusion), transfer the patient to the mortuary for blood wash out, perfusion and then cooling in dry ice
• Heparin is a fast-acting anticoagulant (blood thinner) medication used to prevent and treat blood clots in veins, arteries, the heart, and lungs. It is administered by injection or intravenously because it cannot be absorbed by the digestive system
• Blood Replacement : A perfusion machine takes over the heart's function, pumping a chosen CPA solution into the vascular system to replace blood.
• Gradual CPA Introduction : Perfusion of the head only. This is done using a gravity fed system rather than a mechanical pump. The process begins with a blood washout followed by perfusion. The perfusate is delivered in 6 different concentrations. The steps are overlapped to prevent a sudden change of concentration. The target temperature for the patient during perfusion is -6 to +6 degrees centigrade. The CPA concentration is increased gradually, often with simultaneous cooling, to avoid osmotic damage and cell swelling/shrinkage, ensuring balanced water exchange. A certain amount if tissue shrinkage is unfortunately normal under current protocols. However, we take steps to minimise this such as perfusing slowly and not over-perfusing.
• Vitrification is the process of turning a substance into a glass-like, non-crystalline solid, often by extremely rapid cooling, used in fertility to freeze eggs and embryos by preventing damaging ice crystals. It's a cryopreservation method that solidifies cells into an amorphous state, preserving them for future use with high survival rates.
• Cryoprotectants are substances that protect living cells, tissues, and biological samples from freezing damage by preventing harmful ice crystal formation, allowing survival at low temperatures, and are crucial in medicine (organ banking, cell storage). They work by lowering water's freezing point and reducing ice formation through solute concentration or hydrogen bonding, with common examples including DMSO, glycerol, and ethylene glycol, though they must be non-toxic and ideally penetrate cells
• Dimethyl Sulfoxide Pharmaceutical grade DMSO is highly purified, often 99.9% pure, with contaminants like Dimethyl Sulfide (DMS) removed, making it virtually odorless and suitable for research

• A building with 8-9 metres roof height
• Two patient storage dewars, made to order. One will be the main storage container and a spare in case of leakage. New drawings will be needed as the current designs are for multiple patients.
• A large liquid nitrogen storage tank to feed the dewar as the level drops
• A contract with a liquid nitrogen supplier
• An automated liquid nitrogen top up system
• Back up generators for the top up system
• A nitrogen level monitoring and alarm system for the building
• A winch system to load/unload the dewars
• A nitrogen vapour cool down chamber, made to order. This cools the patient gradually to liquid nitrogen temperature before being placed in the dewar
• A cryonics specialist to set this up correctly

• Cryobiology is the scientific study of how extremely low temperatures affect living organisms, cells, tissues, and biological materials, focusing on preservation techniques like cryopreservation (freezing) to prevent damage from ice formation, using cryoprotectants like glycerol to protect viability for applications in medicine
• The Canadian Wood Frog / Japanese Tree Frog can survive considerable freezing during winter, which in spring can return to normal function. They can go through these freeze and thaw cycles for many years, likely from anti freeze proteins.
• Seperatley a research team from the Japan Advanced Institute published a research paper December 2025 describing an innovative and promising approach to cancer treatment. They report on a bacterial species extracted from the gut of the Japanese Tree Frog that demonstrated a 100% cure rate in a mouse model of human colon cancer. All the untreated mice died; all the treated mice lived, and became resistant to this cancer thereafter. The bacteria (Ewingella americana) were handily cleared from the body of the mice, and they are unlikely to pose a danger to humans when this therapy is translated. Crucially important - the bacteria not only attack the tumor but also enlist the body’s own immune system to aid in the assault.